Mcherry-gfp-lc3双荧光指示

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August undefined, 2024

WebGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统细胞自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至细胞自噬体膜，在荧光显微镜下形成多个明亮的绿色 … Web7 nov. 2015 · Both clones expressed 75kDa mCherry-EGFP-LC3 fusion protein, while clone relativelylow expression level (Fig. 1C). faintband 25kDa detected westernblots may represent free EGFP protein. freeEGFP synthesizeddur- ing translation because EGFPstart stopcodons morelikely producedfrom lysosomal degradation tfLC3B(Hosokawa et al., …

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WebGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统. 自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点 … Web14 jul. 2024 · Metformin treatment and mCherry-GFP-LC3B plasmid transfection showed that metformin could induce the autophagic flux. 3-Methyladenine (3-MA) partly abolished this effect. Conclusion: Metformin could induce the autophagy, autophagic flux, and activate the AMPK-mTOR signaling pathway in human HCC cells. fgbgbn

Autophagy restricts Mycobacterium tuberculosis during acute …

Web17 feb. 2024 · In stable RFP-GFP-LC3 U87 and PC-12 cells, ethyl acetate fraction (EF) was demonstrated to induce the strongest autophagy effect (Supplementary Fig. 2c–e ). After sub-fractionation of EF to 34... Webtandem monomeric mCherry-GFP-LC3 tagged protein, which has been used in in vitro systems to monitor autophagy flux through direct fluorescence microscopy. 9,10 This … fgbfgb fgbfgb

Tracking different stages of autophagy with double-tagged …

mCherry-EGFP-LC3B(Mouse)慢病毒滴度液-细胞自噬-产品中心 …

Web19 feb. 2024 · Tandem constructs have been designed that couple pH-stable fluorophores like RFP or mCherry with the pH sensitive fluorophore, GFP, to monitor LC3 turn over in the lysosome. Attempts to quantify LC3 … WebPremo Autophagy sensors take advantage of autophagy-associated proteins LC3B or p62, along with GFP or RFP, to detect either the induction or inhibition of autophagy in cells. The Premo Autophagy sensors are transduced using BacMam (insect Bac ulovirus with a Mam malian promoter) technology, enabling unambiguous visualization of the chimeric ... fgbfgb ltdWebpDEST-CMV mCherry-GFP-LC3B WT (Plasmid #123230) Print Enlarge View all sequences Purpose Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter. Depositing Lab Robin Ketteler Publication fgbgbgb

"Web(1) an inhibitor of macropinocytosis upregulation, wherein the inhibitor of macropinocytosis upregulation is selected from 5-(N-ethyl-N-isopropyl) amiloride (EIPA), 3-methylsulpho " - Mcherry-gfp-lc3双荧光指示

Mcherry-gfp-lc3双荧光指示

Fluorescent Biosensors for Measuring Autophagic Flux - Addgene

WebmCherry是一种来自于蘑菇珊瑚(mushroom coral)的单体红色荧光蛋白，当其与GFP进行LC3B的共同标记时，在GFP被溶酶体酸性环境所淬灭的情况下，可以发出红色荧光的mCherry因为其卓越的稳定性而被保留。因此，通过融合表达mCherry-GFP-LC3B蛋白，可以非常有效地追踪自噬过程。 Web细胞自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至细胞自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。当细胞自噬溶酶体形成后，酸性的溶酶体环境使GFP荧光淬灭，而mCherry荧光不受影响，细胞自噬溶酶体呈现红色荧光（图6）。

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Web24 okt. 2024 · Careful characterization using LysoTracker and mCherry-LC3 transgenic fish demonstrated this was due to a block in autophagosome degradation rather than an increase in autophagosome formation. A dual GFP-LC3; mCherry-Lamp1 reporter line recently developed by the same group was used to further elucidate the role of lysosome ... WebPlasmid pDEST-CMV mCherry-GFP-LC3B WT from Dr. Robin Ketteler's lab contains the insert Microtubule-associated proteins 1A/1B light chain 3B and is published in …

http://bjbalb.com/html/Apoptosis-Proliferation/YT160.html WebLC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in …

WebpCMV-mCherry-GFP-LC3B是碧云天研发的在哺乳动物细胞中表达红色mCherry和绿色GFP双标签的人源LC3B融合蛋白的质粒。. 该质粒含有CMV启动子，为卡那霉素抗性， … WebTandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. …

WebHepG2 cells were transduced with mCherry-GFP-LC3 lentiviral vectors, which were pre-packed with the lentivirus packaging kit. The quenching properties of the GFP fluorescent signal in acid conditions (pH < 5) and the cherry fluorescent signal do not differ significantly in different acidic environments [ 28 ] when used to detect autophagic flux.

Web8 jul. 2024 · 1. Generation of autophagy reporter cell line of immortalized human chondrocytes. NOTE: The generation of an autophagy reporter cell line by stable transfection of pBABE-mCherry-GFP-LC3 by establishing a flow cytometry quantitative readout was described previously 11.Two cell lines were used in the retroviral … hpsc ado sarkari resultWeb荧光显微镜RFP-GFP-LC3融合蛋白示踪自噬形成使用双荧光自噬慢病毒感染细胞，加入自噬诱导剂后，根据红/绿荧光共定位情况，Merge后的亮黄色荧光点即为自噬体。 3. WB检 … fgbfgbvWebFigure 1A, the detection of mCherry-GFP-LC3-positive dots and the measurement of autophagy flux was reproducible and sensitive, and LC3 was easily detected with different microscopy methods, including direct epifluorescence, confocal microscopy, a super zoom microscope, and spinning-disk microscopy. Using this combinatorial strategy, a robust LC3 hps begumpet alumni