WebTo help you get started with clearing 3D cell cultures, we have clearing validated antibodies. Validated antibodies are for key markers, such as NeuN, GFAP, Iba1, etc, and are tested for tissue clearing with 1 mm mouse brain sections, and for 3D cell culture clearing with neuronal spheroids and hepatic spheroids (depending on the marker). WebWhile there are numerous tissue clearing techniques and versions of these techniques, adopting tissue clearing has been challenging for many researchers as it requires an …

CytoVista™ Tissue Clearing Reagent - Thermo Fisher …

WebCytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. … WebPlace sample on microscope slide and apply sufficient Image-iT™Plant Tissue Clearing Reagent to completely cover the sample. 6. Apply a coverslip and apply gentle heat (50-60°C) until air bubbles move to the edge of the slide. 7. Incubate at room temperature until tissue is visually cleared. siemens nx cad tool

CytoVista Clearing System and ProLong Glass …

WebThe CytoVista Tissue Clearing Kit clears fluorescently labeled tissue to enable sharp and bright 3D fluorescent imaging at any tissue depth. The kit contains both CytoVista … WebJun 19, 2024 · Here, we developed a new Aqueous high refractive Index matching and tissue Clearing solution for Imaging (AICI) for easy application to microscopic imaging. AICI can rapidly clear tissue: it can clear a 1 mm-thick brain slice within 90 min at 35 C. siemens off campus drive