WebDec 6, 2016 · Moreover, this study was the first to reveal the potential lncRNA targets of EZH2 by ChIP sequencing. By integrating ChIP sequencing and co-expression analysis results, we revealed genome-wide shared and tissue/cell specific potential targets, researchers can predict potential targets in a specific cell line or cancer type and the … WebDec 18, 2024 · The development and application of whole genome sequencing technology has greatly broadened our horizons on the capabilities of long non-coding RNAs (lncRNAs). LncRNAs are more than 200 nucleotides in length and lack protein-coding potential. Increasing evidence indicates that lncRNAs exert an irreplaceable role in tumor …
lncRNA-screen: an interactive platform for computationally screening ...
WebFeb 25, 2024 · By inspecting annotated E2F1 ChIP-seq data sets 15, we found that many of the lncRNA transcripts that scored as differentially expressed upon manipulating PRMT5 and/or E2F1 were derived from … WebJun 5, 2024 · ChIP-seq peaks for H3K4me3, H3K27ac and H3K4me1 can be used directly as input to lncRNA-screen. Alternatively, lncRNA-screen can perform its own ChIP-seq analysis starting from raw FASTQ files. Using Botwie2 for alignment and MACS2 for peak calling, histone mark peaks can be identified. Broad peak calling is used (q-value <0.05) … robert ludlum\u0027s the treadstone resurrection
Single-cell RNA sequencing uncovers the nuclear …
WebJun 2, 2015 · CCND1/Cyclin D1 is an lncRNA which is transcribed from the promoter region of the Cyclin D1 gene; ... such as ChIP-Seq [ 65]. The required pipelines can be summarized in three major steps: (i) mapping the reads on the reference genome/transcriptome, (ii) identification of clusters of reads indicating putative RNA … WebOur results uncover the lncRNA PIRAT (PU.1-induced regulator of alarmin transcription) as a major PU.1 feedback-regulator in monocytes, governing the production of the alarmins S100A8/A9, key drivers of COVID-19 … WebDRIPc-seq 平台在使用 S9.6 抗体来富集 DNA:RNA 杂交体后，对杂交体中的 RNA 进行链特异性建库和测序分析，不仅可以提供组织形成 R-loop 的 RNA 信息（比如 mRNA 或 lncRNA ）以及 R-loop 的方向性，还能够实现更精准的 R-loop 定位，并提供半定量信息。 robert ludlum written works