WebJun 24, 2024 · Cell viability was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay obtained from Biofrox (#298-93-1, Germany). For Hoechst fluorescent staining, the cells were fixed with 4% paraformaldehyde at room temperature for 30 min, followed by staining with Hoechst 33258 solution at 37°C for 10 … Webwith CORT for 24 hours. Cell survival was detected by Hoechst staining and MTT assay. The expression of PACAP, cyclic adenosine monophosphate (cAMP) response element binding protein (CREB), and extracellular regulated protein kinases (ERK) were analyzed by western blotting. PACAP RNAi was used to interfere with PC12 cells to downregulate the ...
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WebThe MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. This colorimetric assay is based on the reduction of a yellow tetrazolium salt (3- (4,5-dimethylthiazol-2-yl) … Webdiphenyltetrazolium bromide (MTT) was produced by BioFrox. Dulbecco's modi ed Eagle's medium (DMEM) was bought from GIBCO. Cell ROS testing kits were bought from … WebBioFroxx. 130 likes. BioFroxx products include biochemicals, media and reagents for Life Science applications. BioFroxx is a neoFroxx GmbH Brand. notice ornin d1005